NDUFB11 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2E10]

CAT#: TA807710BM

NDUFB11 mouse monoclonal antibody, clone OTI2E10 (formerly 2E10), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB11 (RC213937, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB11 (1:2000). Positive lysates LY412795 (100ug) and LC412795 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2E10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 30-163 of human NDUFB11(NP_061929) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.2 kDa
• Gene Name: NADH:ubiquinone oxidoreductase subunit B11
• Database Link:
  NP_061929
  Entrez Gene 54539 Human
  UniProt ID Q9NX14
• Background: NDUFB11 is a component of mitochondrial complex I. Complex I catalyzes the first step in the electron transport chain, the transfer of 2 electrons from NADH to ubiquinone, coupled to the translocation of 4 protons across the membrane (Carroll et al., 2002 [PubMed 12381726]). [supplied by OMIM, Feb 2009]. Transcript Variant: This variant (2) uses a different splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter C-terminus when it is compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AY359056.1, BE781599.1 [ECO:0000332] RNAseq introns :: single sample supports all introns ERS025084, ERS025087 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##
• Synonyms: CI-ESSS; ESSS; Np15; NP17.3; P17.3

Reference Data

• Protein Families: Transmembrane