NDUFB11 Mouse Monoclonal Antibody [Clone ID: OTI8C5]

CAT#: TA807693S

NDUFB11 mouse monoclonal antibody, clone OTI8C5 (formerly 8C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB11 (RC213937, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB11. Positive lysates LY412795 (100ug) and LC412795 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 5 different cell lines and 4 human tissue by using anti-NDUFB11 monoclonal antibody (1: Hela; 2: HepG2; 3: K562; 4: Mcf7; 5: SVT2; 6: Liver; 7: Testis; 8: Brain; 9: Uterus) at 1:200 dilution.

Specifications

Product Data

• Clone Name: OTI8C5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 30-163 of human NDUFB11(NP_061929) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.2 kDa
• Gene Name: NADH:ubiquinone oxidoreductase subunit B11
• Database Link:
  NP_061929
  Entrez Gene 54539 Human
  UniProt ID Q9NX14
• Background: NDUFB11 is a component of mitochondrial complex I. Complex I catalyzes the first step in the electron transport chain, the transfer of 2 electrons from NADH to ubiquinone, coupled to the translocation of 4 protons across the membrane (Carroll et al., 2002 [PubMed 12381726]). [supplied by OMIM, Feb 2009]
• Synonyms: CI-ESSS; ESSS; Np15; NP17.3; P17.3

Reference Data

• Protein Families: Transmembrane