GARS1 Mouse Monoclonal Antibody [Clone ID: OTI6C5]

CAT#: TA805168S

GARS mouse monoclonal antibody, clone OTI6C5 (formerly 6C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GARS (Cat# RC202852, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GARS(Cat# TA805168). Positive lysates LY419568 (100ug) and LC419568 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805168)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805168)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805168)

Specifications

Product Data

• Clone Name: OTI6C5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GARS (NP_002038) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83 kDa
• Gene Name: glycyl-tRNA synthetase
• Database Link:
  NP_002038
  Entrez Gene 297113 RatEntrez Gene 353172 MouseEntrez Gene 2617 Human
  UniProt ID P41250
• Background: This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. [provided by RefSeq, Jul 2008]
• Synonyms: CMT2D; DSMAV; GlyRS; HMN5; SMAD1

Reference Data

• Protein Pathways: Aminoacyl-tRNA biosynthesis