HIC5 (TGFB1I1) Rabbit Polyclonal Antibody

CAT#: TA590254

Rabbit Polyclonal HIC5 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TGFB1I1 (Cat# RC200761, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGFB(Cat# TA590254). Positive lysates LY402475 (100ug) and LC402475 (20ug) can be purchased separately from OriGene.

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  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

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  ELISA: HIC5 Antibody

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TGFB1I1 rabbit polyclonal antibody. (TA590254)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-TGFB1I1 rabbit polyclonal antibody. (TA590254)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TGFB1I1 rabbit polyclonal antibody. (TA590254)

• Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in parietal cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

• Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm & focal adhesions.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

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Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the Middle Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 1.25547 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: transforming growth factor beta 1 induced transcript 1
• Database Link:
  NP_057011
  Entrez Gene 7041 Human
  UniProt ID O43294
• Background: HIC5 functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane henceregulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity.
• Synonyms: ARA55; HIC-5; HIC5; TSC-5
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Families: Druggable Genome, Transcription Factors