DDT Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3B10]

CAT#: TA505591BM

DDT mouse monoclonal antibody,clone 3B10, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDT (RC202047, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDT. Positive lysates LY419969 (100ug) and LC419969 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DDT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-DDT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505591)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DDT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505591)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505591)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DDT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505591)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DDT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505591)

• Immunofluorescent staining of HeLa cells using anti-DDT mouse monoclonal antibody (TA505591) (1:100).

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Specifications

Product Data

• Clone Name: OTI3B10
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DDT(NP_001346) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.5 kDa
• Gene Name: D-dopachrome tautomerase
• Database Link:
  NP_001346
  Entrez Gene 1652 Human
  UniProt ID P30046
• Background: D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq]
• Synonyms: DDCT