HDAC6 Mouse Monoclonal Antibody [Clone ID: OTI3G7]

CAT#: TA502083S

HDAC6 mouse monoclonal antibody, clone OTI3G7 (formerly 3G7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC6 (RC209649, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC6. Positive lysates LY401822 (100ug) and LC401822 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.

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  Anti-HDAC6 mouse monoclonal antibody (TA502083) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC6 (RC209649).

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  HEK293T cells transfected with either RC209649 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDAC6 antibody (TA502083), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI3G7
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HDAC6 (NP_006035) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.81 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 131.2 kDa
• Gene Name: histone deacetylase 6
• Database Link:
  NP_006035
  Entrez Gene 10013 Human
  UniProt ID Q9UBN7
• Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq]
• Synonyms: CPBHM; HD6; JM21; PPP1R90

Reference Data

• Protein Families: Druggable Genome, Transcription Factors