rpmB Rabbit Polyclonal Antibody

CAT#: TA398701

rpmB/L28 Antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB: 0.5 μg/mL.
  
  Antibody validated: Western Blot in E.coli samples. All other applications and species not yet tested.
• Reactivities: Escherichia coli
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Anti-rpmB/L28 antibody (10061) was raised against an 15 amino acid peptide near the amino terminus of E. coli rpmB/L28.
  
  The immunogen is located within the first 50 amino acids of rpmB/L28.
• Formulation: rpmB/L28 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Purification: rpmB/L28 Antibody is affinity chromatography.
• Conjugation: Unconjugated
• Storage: rpmB/L28 Antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 9 kD
  
  Observed: 9 kD
• Database Link:
  UniProt ID P0A7M2
• Background: The rpmB/L28 protein is a component of the 50S subunit of the ribosome and is required for ribosome assembly. rpmB/L28 interacts with 23S rRNA and crosslinks to L9. L15 and L17 stimulate the interactions of rpmB/L28 with 23S rRNA. rpmB/L28 is phosphorylated at Thr8. rpmB/L28 is labeled by the macrolides carbomycin A, niddamycin and tylosin, which inhibit ribosomal activity.
• Note: Optimal dilutions for each application to be determined by the researcher.