FYN Rabbit Polyclonal Antibody

CAT#: TA392891

Fyn (phospho-Y530) polyclonal antibody

Product Images

Western blot (WB) analysis of p-Fyn (Y530) pAb at 1:1000 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:A375 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug) Lane6:C6 whole cell lysate(40ug) Lane7:CT-26 whole cell lysate(40ug)

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  Immunohistochemistry (IHC) analyzes of p-Fyn (Y530) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Fyn around the phosphorylation site of Tyrosine 530.
• Specificity: p-Fyn (Y530) polyclonal antibody detects endogenous levels of Fyn protein only when phosphorylated at Tyr530.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 59 kDa
• Gene Name: FYN proto-oncogene, Src family tyrosine kinase
• Database Link:
  Entrez Gene 2534 Human
  UniProt ID P06241
• Background: Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.
• Synonyms: FYN; p59-Fyn; Proto-oncogene c-Fyn; Proto-oncogene Syn; SLK; Src-like kinase; Tyrosine-protein kinase Fyn
• Note: For research use only, not for use in diagnostic procedure.