p38 (CRK) Rabbit Polyclonal Antibody

CAT#: TA392884

Crk2 (phospho-Y221) polyclonal antibody

Product Images

Western blot (WB) analysis of Crk2 (phospho-Y221) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:The Kidney tissue lysate of Rat(40ug) Lane5:The Kidney tissue lysate of Mouse(40ug)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Crk2 around the phosphorylation site of Tyrosine 221.
• Specificity: Crk2 (phospho-Y221) polyclonal antibody detects endogenous levels of Crk2 protein only when phosphorylated at Tyr221.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 34 kDa
• Gene Name: v-crk avian sarcoma virus CT10 oncogene homolog
• Database Link:
  Entrez Gene 1398 Human
  UniProt ID P46108
• Background: Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway.
• Synonyms: Adapter molecule crk; CRK; CRKII; p38 CRK; Proto-oncogene c-Crk
• Note: For research use only, not for use in diagnostic procedure.