RICTOR Rabbit Polyclonal Antibody

CAT#: TA392544

Rictor (Phospho-Thr1135) polyclonal antibody

Product Images

Western blot (WB) analysis of Rictor (Phospho-Thr1135) polyclonal antibody at 1:500 dilution Lane1:Hela treated with λ-phosphatase whole cell lysate(40ug) Lane2:HeLa treated with IGF-1(50 ng/ml,30 minutes) whole cell lysate(40ug) Lane3:HeLa treated with IGF-1(50 ng/ml,15 minutes) whole cell lysate(40ug) Lane4:HeLa whole cell lysate(40ug)

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  Immunohistochemistry of paraffin-embedded Rat Heart using Rictor (Phospho-Thr1135) antibody at dilution of 1:50.

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  Immunofluorescence analysis of Hela cells using Rictor antibody at dilution of 1:50.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Rictor around the phosphorylation site of Threonine 1135.
• Specificity: Rictor (Phospho-Thr1135) polyclonal antibody detects endogenous levels of Rictor protein only when phosphorylated at Thr1135.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 210 kDa
• Gene Name: RPTOR independent companion of MTOR complex 2
• Database Link:
  Entrez Gene 253260 Human
  UniProt ID Q6R327
• Background: Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals. mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor. The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro. This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes. This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR. Phosphorylation of Thr1135 on rictor was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery. Additional research indicates that rictor is phosphorylated at Thr1135 by p70 S6K, which negatively regulates mTORC2 protein complex as part of a negative feedback mechanism controlling Akt activity.
• Synonyms: AVO3 homolog; hAVO3; KIAA1999; Rapamycin-insensitive companion of mTOR; RICTOR
• Note: For research use only, not for use in diagnostic procedure.