GOLGA2 Mouse Antibody [Clone ID: M342]

CAT#: TA389142

Anti-GM130 (C-terminal region) Antibody

Specifications

Product Data

• Clone Name: M342
• Applications: ICC, WB
• Recommended Dilution: WB: 1:500
  ICC: 1:50
• Reactivities: Human, Rat, Mouse
• Host: Mouse
• Isotype: IgG1
• Immunogen: Clone M342 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human GM130. This sequence has high homology to similar regions in rat and mouse GM130.
• Specificity: This antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of GM130 on SDS-PAGE immunoblots of mouse C2C12 cells. In immunocytochemistry, anti-GM130 specifically stains Golgi in mouse C2C12 cells.
• Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
• Concentration: lot specific
• Purification: Protein A Purified
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 130
• Database Link:
  UniProt ID Q08379
• Background: Golgi Matrix Protein (GM130) is a peripheral cytoplasmic protein that is bound to Golgi membranes. It maintains cis-Golgi structure, and it regulates the disassembly and reassembly of the Golgi complex during mitosis. GM130 may also be important during docking and fusion of coatomer (COPI) coated vesicles to the Golgi membrane. The carboxy-terminal domain of GM130 is highly homologous to the human auto-antigen, golgin-95. GM130 interacts in a GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes. It has also been implicated in the activation of Ste-kinase, YSK1, during the golgi reorganization that occurs along with cell migration.
• Note: Protein G purified tissue culture supernatant.