Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: LN6]

CAT#: TA389037

Anti-Vimentin Antibody

Specifications

Product Data

• Clone Name: LN6
• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Cell nuclei preparation from adult human thymus.
• Specificity: Specific for the ~50 kDa Vimentin protein.
• Formulation: 100 µl in PBS
• Concentration: lot specific
• Purification: Protein L Purified
• Conjugation: Unconjugated
• Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 50
• Gene Name: vimentin
• Database Link:
  Entrez Gene 7431 Human
  UniProt ID P08670
• Background: Vimentin is the major protein subunit of the 10 nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).
• Synonyms: FLJ36605; vimentin
• Note: Protein L purified culture supernatant