Phospho-Mecp2 (pSer421) Rabbit Polyclonal Antibody

CAT#: TA389019

Anti-MeCP2 (Ser421) Antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000
  WB Brain: 1:1000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 of mouse MeCP2, conjugated to keyhole limpet hemocyanin (KLH).
• Specificity: Specific for endogenous levels of the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
• Formulation: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Concentration: lot specific
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 55
• Gene Name: methyl CpG binding protein 2
• Database Link:
  Entrez Gene 17257 Mouse
  UniProt ID P51608
• Background: MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser-421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment (Hutchinson et al., 2012).
• Synonyms: AUTSX3; DKFZp686A24160; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT
• Note: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.