Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: 6G3]

CAT#: TA389013

Anti-Ki-67 Antibody

Specifications

Product Data

• Clone Name: 6G3
• Applications: ICC, IHC, WB
• Recommended Dilution: WB: 1:2000
  ICC: 1:2000-1:5000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant construct containing the N-terminal 300 amino acids of the human sequence expressed in and purified from E. coli.
• Specificity: Specific for endogenous levels of the ~345 & 395 kDa Ki-67 isoforms. Additional bands are thought to be breakdown products of these 2 isoforms.
• Formulation: PBS + 50% glycerol and 5 mm NaN3
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 345-395
• Gene Name: marker of proliferation Ki-67
• Database Link:
  Entrez Gene 4288 Human
  UniProt ID P46013
• Background: Ki-67 is a nuclear protein that is considered to be essential for cellular proliferation since it is expressed only in active phases of the cell cycle (G1, S, G2 and mitosis) but not in resting phase G0 (Scholzen & Gerdes 2000). Ki-67 antibodies have therefore been widely used as markers of cell growth. Ki-67 has further been identified as a predicative and prognostic marker in breast, lung, cervical and other cancers (Yerushalmi et al., 2010; Folescu et al., 2018; Das et al., 2018).
• Synonyms: KI67; KIA
• Note: Protein G purified culture supernatant