SM22 alpha (TAGLN) Rabbit Monoclonal Antibody [Clone ID: SAIC-33B-129]

CAT#: TA385906

Recombinant Anti-TAGLN (Clone SAIC-33B-129)

Product Images

Flow cytometry using the antibody SAIC-33B-129 (TA385906). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (TA385792; isotype control, black line) or the rabbit IgG version of SAIC-33B-129 (TA385906, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• 

  Western Blot using anti-TAGLN antibody SAIC-33B-129 (TA385906). HeLa(A), Caco-2(B), U2OS(C) cell lysate and human placenta(D), muscle(E) tissue lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-33B-129 (TA385906) at 0.0003 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: SAIC-33B-129
• Applications: ELISA, WB
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Peptide "AAEDYGVIK", derived from the actin cross-linking protein Transgelin, conjugated to KLH.
• Specificity: Recognises human TAGLN.
  
  Original data characterizing this antibody may be found here .
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: transgelin
• Database Link:
  Entrez Gene 6876 Human
  UniProt ID Q01995
• Synonyms: SM22; Sm22; Sm22a; Ws310