SNF2H (SMARCA5) Rabbit Monoclonal Antibody [Clone ID: R05-9D5]

CAT#: TA385281

SMARCA5 Rabbit monoclonal Antibody

Product Images

Western blot analysis of SNF2H in K562, C6, 3T3, Hela lysates using SNF2H antibody.

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  Immunocytochemistry analysis of SMARCA5 (green) in Jurkat using SMARCA5 antibody,and DAPI(blue).

Specifications

Product Data

• Clone Name: R05-9D5
• Applications: IF, WB
• Recommended Dilution: WB: 1/1000
  ICC/IF: 1/50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human SNF2H
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 122 kDa; Observed MW: 122 kDa
• Gene Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
• Database Link:
  Entrez Gene 8467 Human
  UniProt ID O60264
• Background: Swiss-Prot Acc.O60264.Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.
• Synonyms: hISWI; hSNF2H; ISWI; SNF2H; WCRF135