Lin28 (LIN28A) Mouse Monoclonal Antibody [Clone ID: 9G2-5B6-1D1]

CAT#: TA384667

LIN28A (9G2) Mouse monoclonal Antibody

Product Images

Confocal immunofluorescence analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.

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  Confocal immunofluorescence analysis of NTERA-2 cells using LIN28 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

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  Western blot analysis using LIN28 mouse mAb against NTERA-2 cell lysate (1).

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  Western blot analysis of LIN28A (9G2) in NTERA-2 lysates using LIN28A (9G2) antibody.

Specifications

Product Data

• Clone Name: 9G2-5B6-1D1
• Applications: IF, WB
• Recommended Dilution: WB: 1/500-1/2000
  ICC: 1/200-1/1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli.
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Ascitic Fluid
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 23kDa
• Gene Name: lin-28 homolog A
• Database Link:
  Entrez Gene 79727 Human
  UniProt ID Q9H9Z2
• Background: Swiss-Prot Acc.Q9H9Z2.LIN28: lin-28 homolog (C. elegans), also known as CSDD1, ZCCHC1. Entrez Protein NP_078950. LIN28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.
• Synonyms: AL024421; ENSMUSG00000070700; Gm10299; Lin-28; lin-28A; Lin28; RGD1566408; Tex17