Raptor (RPTOR) Rabbit Polyclonal Antibody

CAT#: TA381147

Phospho-Raptor-Ser792 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of 293T cells, using Phospho-Raptor-Ser792 antibody (TA381147) at 1:1000 dilution.293T cells were treated by Oligomycin (0.5 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1S.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse
• Modifications: Phospho Ser792
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding Ser792 of human RPTOR
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 43kDa/131kDa/149kDa
• Gene Name: regulatory associated protein of MTOR complex 1
• Database Link:
  Entrez Gene 57521 Human
  UniProt ID Q8N122
• Background: This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
• Synonyms: KIAA1303; KOG1; Mip1; RAPTOR