OGA Rabbit Polyclonal Antibody

CAT#: TA378511

MGEA5 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using MGEA5 antibody (TA378511) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:200 - 1:2000
  IHC,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-580 of human MGEA5 (NP_001135906.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 76kDa/95kDa/96kDa/102kDa
• Gene Name: meningioma expressed antigen 5 (hyaluronidase)
• Database Link:
  Entrez Gene 10724 Human
  UniProt ID O60502
• Background: The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
• Synonyms: FLJ11229; FLJ23355; HEXC; KIAA0679; MEA5; NCOAT; O-GlcNAcase; OGA