KLF4 Rabbit Polyclonal Antibody

CAT#: TA377841

KLF4 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using KLF4 antibody (TA377841) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of U2OS cells using KLF4 Rabbit pAb (TA377841) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-479 of human KLF4 (NP_004226.3).
• Formulation: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 6kDa/12kDa/45kDa/51kDa/54kDa
• Gene Name: Kruppel-like factor 4 (gut)
• Database Link:
  Entrez Gene 9314 Human
  UniProt ID O43474
• Background: This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Synonyms: EZF; GKLF