Fadd Rabbit Polyclonal Antibody

CAT#: TA376076

FADD Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of Mouse heart, using FADD antibody (TA376076) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Western blot analysis of extracts of Jurkat cells, using FADD antibody (TA376076) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

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  Immunoprecipitation analysis of 600ug extracts of Mouse heart cells using 3ug FADD antibody (TA376076). Western blot was performed from the immunoprecipitate using FADD antibody (TA376076) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:200 - 1:2000
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human FADD.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: Fas (TNFRSF6)-associated via death domain
• Database Link:
  Entrez Gene 14082 Mouse
  UniProt ID Q61160
• Background: Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95 or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.
• Synonyms: GIG3; MGC8528; MORT1