ESE1 (ELF3) Rabbit Polyclonal Antibody

CAT#: TA375851

ESE1/ELF3 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ESE1/ESE1/ELF3 antibody (TA375851) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug ESE1/ESE1/ELF3 antibody (TA375851). Western blot was performed from the immunoprecipitate using ESE1/ESE1/ELF3 antibody (TA375851) at a dilition of 1:500.____Detection: ECL Enhanced Kit ._Exposure time: 30s.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IP,1:20 - 1:50
• Reactivities: Human, Mouse
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ESE1/ESE1/ELF3 (NP_004424.3).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 39kDa/41kDa
• Gene Name: E74 like ETS transcription factor 3
• Database Link:
  Entrez Gene 1999 Human
  UniProt ID P78545
• Background: Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development (By similarity.
• Synonyms: EPR-1; ERT; ESE-1; ESX; JEN; OTTHUMP00000034052