EIF3D Rabbit Polyclonal Antibody

CAT#: TA375808

EIF3D Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using EIF3D antibody (TA375808) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of L-929 cells using EIF3D antibody (TA375808) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of C6 cells using EIF3D antibody (TA375808) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L-929 cells using EIF3D antibody (TA375808) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using EIF3D antibody (TA375808) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:200 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF3D (NP_003744.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 58kDa/62kDa/63kDa
• Gene Name: eukaryotic translation initiation factor 3 subunit D
• Database Link:
  Entrez Gene 8664 Human
  UniProt ID O15371
• Background: Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex.
• Synonyms: eIF-3-zeta; eIF3-p66; eIF3-zeta; EIF3S7; MGC17258; MGC126526; OTTHUMP00000197901