DARS1 Rabbit Polyclonal Antibody

CAT#: TA375324

DARS Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using DARS Antibody (TA375324) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug DARS antibody (TA375324). Western blot was performed from the immunoprecipitate using DARS antibody (TA375324) at a dilition of 1:1000.

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human DARS (NP_001340.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 45kDa/57kDa
• Gene Name: aspartyl-tRNA synthetase
• Database Link:
  Entrez Gene 1615 Human
  UniProt ID P14868
• Background: This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants.
• Synonyms: AspRS; DKFZp781B11202; MGC111579