Aminomethyltransferase (AMT) Rabbit Polyclonal Antibody

CAT#: TA373495

AMT Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using AMT antibody (TA373495) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of HeLa cells using AMT Rabbit pAb (TA373495) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH/3T3 cells using AMT Rabbit pAb (TA373495) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 107-386 of human AMT (NP_001158184.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 37kDa/39kDa/41kDa/43kDa
• Gene Name: aminomethyltransferase
• Database Link:
  Entrez Gene 275 Human
  UniProt ID P48728
• Background: This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: aminomethyltransferase; GCE; GCST; GCVT; NKH