STUB1 Rabbit Polyclonal Antibody

CAT#: TA356314

STUB1 Antibody - C-terminal region

Product Images

WB Suggested Anti-STUB1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: Transfected 293T

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  Lanes: 1:1ug insoluble STUB1 protein, 2:1ug soluble STUB1 protein, 3:1ug EPM2A protein, 4:1ug insoluble PPP1R3°C protein, 5:1ug soluble PPP1R3°C protein Primary Antibody Dilution: 1:2500 Secondary Antibody: Anti-rabbit-AP Secondary Antibody Dilution: 1:20,000 Gene Name: STUB1 Submitted by: Pedro Castanheira, Biocant

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  Lanes: Lane 1: 40ug canine retina lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Goat anti-rabbit-HRP Secondary Antibody Dilution: 1:5000 Gene Name: STUB1 Submitted by: Dr. Stefania Marsili and Dr. William Beltran, University of Pennsylvania

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  Rabbit Anti-STUB1 Antibody Catalog Number: TA356314 Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic Primary Antibody Concentration: N/A Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5–2.0 sec

Specifications

Product Data

• Applications: IHC, WB
• Reactivities: Human, Dog
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human STUB1
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish
  Homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 93%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  Note that this product is shipped as lyophilized powder to China customers.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 35kDa
• Gene Name: STIP1 homology and U-box containing protein 1
• Database Link:
  NP_005852
  Entrez Gene 479892 DogEntrez Gene 10273 Human
  UniProt ID Q9UNE7
• Background: STUB1 modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It has E3 ubiquitin-protein ligase activity and targets misfolded chaperone substrates towards proteasomal degradation. STUB1 mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation.
• Synonyms: CHIP; HSPABP2; NY-CO-7; SDCCAG7; UBOX1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis