FAM3C Rabbit Polyclonal Antibody

CAT#: TA336975

Rabbit Polyclonal FAM3C Antibody

Product Images

Simple Western: FAM3C Antibody TA336975 - Simple Western lane view shows a specific band for FAM3C in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Western Blot: FAM3C Antibody TA336975 - Analysis of FAM3C in human brain lysate (0.5 ug/ml) 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate (0.5 ug/ml), and 4) in rat brain lysate (0.5 ug/ml) using FAM3C antibody.

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  Western Blot: FAM3C Antibody TA336975 - Total protein from human testis tissue and mouse testis tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FAM3C in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

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  Immunohistochemistry-Paraffin: FAM3C Antibody TA336975 - Analysis of FAM3C in human skin using FAM3C antibody at 10 ug/ml.

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  Immunohistochemistry-Paraffin: FAM3C Antibody TA336975 - Staining of FAM3C using FAM3C antibody in normal human brain at 5 ug/ml (using control rabbit Ig for figure B).

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  Immunohistochemistry-Paraffin: FAM3C Antibody TA336975 - Analysis of tissue section of mouse brain using FAM3C antibody at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles.

• Immunohistochemistry-Paraffin: FAM3C Antibody TA336975 - Analysis tissue section of mouse brain using FAM3C antibody Lot A1 at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles.

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Specifications

Product Data

• Applications: IHC, Simple Western, WB
• Recommended Dilution: Simple Western: 1:20, Western Blot: 0.5-3 ug/ml, Immunohistochemistry: 1:200, Immunohistochemistry-Paraffin: 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A portion of amino acids 40-80 of human FAM3C was used as the immunogen for this antibody.
• Formulation: PBS, 30% glycerol, 0.1% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: family with sequence similarity 3 member C
• Database Link:
  NP_055703
  Entrez Gene 27999 MouseEntrez Gene 312159 RatEntrez Gene 10447 Human
  UniProt ID Q92520
• Synonyms: GS3786; ILEI

Reference Data

• Protein Families: Secreted Protein, Transmembrane