TEM8 (ANTXR1) Mouse Monoclonal Antibody [Clone ID: 200C1339(SB20)]

CAT#: TA336418

Mouse Monoclonal TEM8/ANTXR1 Antibody (200C1339(SB20))

Product Images

Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) TA336418 - Human colon stained with TEM8 antibody at 2.5 ug/ml concentration using DAB chromogen detection method (10X magnification)

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  Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) TA336418 - Detection of TEM8/ANTXR1 in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was observed in the cancerous cells, while the blood vessels depicted the expected intense positivity for TEM8.

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  Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) TA336418 - Detection in TEM8 transfected cell lysate using this antibody.

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  Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) TA336418 - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody (TA336418) at 0.3ug/ml. Band observed at 65kDa. (Expected MW of 62.8kDa according to NP_115584.1).

Specifications

Product Data

• Clone Name: 200C1339(SB20)
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1-3 ug/ml, Immunohistochemistry: 2-5 ug/ml, Immunohistochemistry-Paraffin: 2-5 ug/ml, Immunocytochemistry/ Immunofluorescence)
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: anthrax toxin receptor 1
• Database Link:
  NP_444262
  Entrez Gene 84168 Human
  UniProt ID Q9H6X2
• Background: Recently, using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al, have identified 46 genes, whose expression is specifically elevated in tumor-associated endothelium. Nine of these genes were prominently expressed only in tumor endothelial cells (EC), but were absent or barely detectable in normal ECs, and named as Tumor Endothelial Markers (TEMs, TEM 1-9). Based on TEM8s interaction with the C5 domain of collagen a3(VI), a protein also preferentially expressed in tumor endothelium, it is thought that TEM8 plays an important role in tumor angiogenesis.
• Synonyms: ATR; GAPO; TEM8
• Note: Immunohistochemistry-Paraffin: see Hotchkiss et al 2005 for details. Immunocytochemistry/Immunofluorescence: See Matsuda (2010) for details.

Reference Data

• Protein Families: Druggable Genome, Transmembrane