HIF1 beta (ARNT) Goat Polyclonal Antibody

CAT#: TA326734

Goat Anti-ARNT (aa69-82) Antibody

Product Images

TA326734 (2ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:4000; WB: 2-4µg/ml
• Reactivities: Human (Expected from sequence similarity: Mouse, Rat, Dog, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence CNDKERFARSDDEQS, from the internal region (near N Terminus) of the protein sequence according to NP_001659.1;.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: aryl hydrocarbon receptor nuclear translocator
• Database Link:
  NP_001659
  Entrez Gene 11863 MouseEntrez Gene 25242 RatEntrez Gene 483185 DogEntrez Gene 405 Human
  UniProt ID P27540
• Synonyms: bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO
• Note: Approx 90kDa band observed in nuclear lysates of cell lines HeLa and HepG2 (calculated MW of 86.6kDa according to NP_001659.1). Recommended concentration: 2-4µg/ml. This antibody is expected to recognize all reported isoform 1 (NP_001659.1) only.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma