Pellino 1 (PELI1) Rabbit Polyclonal Antibody

CAT#: TA326634

Rabbit Polyclonal Pellino 1 Antibody

Product Images

Western blot analysis of Pellino 1 in human liver tissue lysate with Pellino 1 antibody at (A) 1 and (B) 2 ug/mL.

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  Immunocytochemistry of Pellino in HepG2 cells with Pellino 1 antibody at 2.5 ug/ml.

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  Immunofluorescence of Pellino 1 in HepG2 cells with Pellino 1 antibody at 20 ug/ml.

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  Immunocytochemistry of Pellino in HepG2 cells with Pellino 1 antibody at 2.5 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Rabbit polyclonal Pellino 1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Pellino 1.
• Formulation: Pellino 1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Pellino 1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46 kDa
• Gene Name: pellino E3 ubiquitin protein ligase 1
• Database Link:
  NP_065702
  Entrez Gene 67245 MouseEntrez Gene 305549 RatEntrez Gene 57162 Human
  UniProt ID Q96FA3
• Background: Pellino 1 Antibody: The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways that lead to activation of MAP kinases and transcription factors. Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex, ultimately resulting in the activation of NF-κB. Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase, able to catalyze the polyubiquitination of IRAK1. It is activated via phosphorylation by either IRAK1 and IRAK4 or the IKK-related kinases IKK-ε and TBK1. In addition to phosphorylation, Pellino 1 activity is also modulated via ubiquitination and sumoylation.
• Synonyms: DKFZp686C18116; MGC50990