SORBS2 Rabbit Polyclonal Antibody

CAT#: TA324888

Rabbit polyclonal ARGBP2 Antibody (N-term)

Product Images

Western blot analysis of ARGBP2 Antibody (N-term) (Cat. #TA324888) in MDA-MB231 cell line lysates (35ug/lane). ARGBP2 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ARGBP2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of U251 cell stained with ARGBP2 Antibody (N-term) (Cat#TA324888).U251 cells were incubated with ARGBP2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).ARGBP2 immunoreactivity is localized to Nucleus significantly.

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  Flow cytometric analysis of MDA-231 cells using ARGBP2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000, IHC: 1:10~50, FC: 1:10~50
• Reactivities: Human (Predicted: Mouse, Rat, Pig)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This ARGBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-204 amino acids from the N-terminal region of human ARGBP2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 124108 Da
• Gene Name: sorbin and SH3 domain containing 2
• Database Link:
  NP_066547
  Entrez Gene 114901 RatEntrez Gene 234214 MouseEntrez Gene 8470 Human
  UniProt ID O94875
• Synonyms: ARGBP2; PRO0618