RNF20 Rabbit Polyclonal Antibody

CAT#: TA319965

Rabbit Polyclonal RNF20 Antibody

Product Images

Western blot analysis of RNF20 in human liver tissue lysate with RNF20 antibody at (A) 1 and (B) 2 ug/mL.

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  Immunohistochemistry of RNF20 in human liver tissue with RNF20 antibody at 2.5 ug/mL.

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  Immunofluorescence of RNF20 in human liver tissue with RNF20 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: RNF20 antibody was raised against a 19 amino acid peptide near the amino terminus of human RNF20.
• Formulation: RNF20 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: RNF20 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 99, 107 kDa; Observed: 100, 110 kDa
• Gene Name: ring finger protein 20
• Database Link:
  NP_062538
  Entrez Gene 109331 MouseEntrez Gene 56254 Human
  UniProt ID Q5VTR2
• Background: RNF20 Antibody: RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.
• Synonyms: BRE1; BRE1A; hBRE1

Reference Data

• Protein Families: Druggable Genome