Il27 Rat Monoclonal Antibody [Clone ID: 3H12.F10]

CAT#: TA319601

Rat monoclonal Mouse IL-27/P28 antibody

Product Images

Anti-IL-27/p28 antibody in WB shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 ug) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was incubated with anti-IL-27/p28 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000.

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  Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10ng/mL IFN? and 1ug/mL LPS for 14 hours and incubated for 4 hours with Bredfeldin A. Cells were stained with biotinylated primary anti-mouse p28 antibody at (0.1 - 10ug/mL primary antibody alongside negative controls of unstimulated cells and isotype controls. Cells were stained with 0.25ug/mL rat anti-mouse CD107b conjugated Alexa Fluor 647 and PHYCOERYTHRIN Conjugated secondary at 1:100.

Specifications

Product Data

• Clone Name: 3H12.F10
• Applications: FC, WB
• Recommended Dilution: ELISA: 1:10,000, WB: 1:1000
• Reactivities: Mouse
• Host: Rat
• Clonality: Monoclonal
• Immunogen: This Protein A purified monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interleukin 27
• Database Link:
  NP_663611
  Entrez Gene 246779 Mouse
  UniProt ID Q8K3I6
• Synonyms: IL-27; IL-27A; IL27-A; IL27A; IL27p28; IL30; MGC71873; p28
• Note: Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.