Cyclin B1 (CCNB1) Rabbit Polyclonal Antibody

CAT#: TA319230

Rabbit polyclonal Cyclin B1 phospho S126 antibody

Product Images

WB using Anti-Cyclin B pS126 antibody shows detection of a band ~48 kDa corresponding to phosphorylated human Cyclin B (arrowheads) in various whole cell lysates. Lysates tested were lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). The primary antibody was used at 1:100. HRP conjugated Gt-a-Rabbit IgG [H&L] was used at 1:5000.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:50,000, WB: 1:100 - 1:1,000, IP: 1:100
• Reactivities: Human, Mouse, Dog, Hamster, Rat, Chimpanzee
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 120-131 of Human Cyclin B1 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cyclin B1
• Database Link:
  NP_114172
  Entrez Gene 25203 RatEntrez Gene 268697 MouseEntrez Gene 608420 DogEntrez Gene 891 Human
  UniProt ID P14635
• Synonyms: CCNB
• Note: Cyclin B1 (also called CCNB1 and G2/mitotic-specific cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form a serine/threonine kinase holoenzyme complex called the maturation-promoting factor (MPF). Two alternative transcripts have been found a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. S126 phosphorylation, the site of phosphorylation recognized by this antibody, is involved in an auto-phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation