PRAK (MAPKAPK5) Rabbit Polyclonal Antibody

CAT#: TA308640

Rabbit polyclonal antibody to PRAK (mitogen-activated protein kinase-activated protein kinase 5)

Product Images

Sample (30 ug of whole cell lysate). A:293T. B:H1299. 7.5% SDS PAGE. TA308640 diluted at 1:500

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PRAK (TA308640) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human (Predicted: Mouse, Chicken, Pig, Rat, X. tropicalis)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment contain a sequence corresponding to a region within amino acids 197 and 457 of PRAK (Uniprot ID#Q8IW41)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54 kDa
• Gene Name: mitogen-activated protein kinase-activated protein kinase 5
• Database Link:
  NP_620777
  Entrez Gene 17165 MouseEntrez Gene 498183 RatEntrez Gene 8550 Human
  UniProt ID Q8IW41
• Background: The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]
• Synonyms: MAPKAP-K5; MK-5; MK5; PRAK
• Note: Seq homology of immunogen across species: Mouse (96%), Chicken (95%), Pig (97%), Rat (96%), Xenopus tropicalis (90%)

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway