Rptor Rabbit Polyclonal Antibody

CAT#: TA306211

Rabbit Polyclonal Raptor Antibody

Product Images

Western blot analysis of Raptor in L1210 cell lysate with Raptor antibody at (A) 2 and (B) 4 ug/ml.

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  Immunohistochemistry of Raptor in L1210 cells with Raptor antibody at 10 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 2 - 4 ug/mL, ICC: 10 ug/mL
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Raptor (CT) polyclonal antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of mouse Raptor.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: regulatory associated protein of MTOR, complex 1
• Database Link:
  NP_083174
  Entrez Gene 74370 Mouse
• Background: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 2). Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation (3). These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels (4). Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins (5).
• Synonyms: KIAA1303; KOG1; Mip1; RAPTOR