Diablo Rabbit Polyclonal Antibody

CAT#: TA306067

Rabbit Polyclonal Smac Antibody

Specifications

Product Data

• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: WB: 1 µg/mL; IF: 10 µg/mL; IHC: 2 µg/mL.
  Antibody validated: Western Blot in mouse and rat samples; Immunofluorescence and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Smac antibody was raised against a peptide corresponding to amino acids 222 to 237 of murine Smac/DIABLO.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Smac Antibody is DEAE purified.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: diablo, IAP-binding mitochondrial protein
• Database Link:
  AF203914
  Entrez Gene 66593 Mouse
  UniProt ID Q9JIQ3
• Background: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).
• Synonyms: DFNA64; SMAC