ISG15 Rabbit Polyclonal Antibody

CAT#: TA302035

Rabbit Polyclonal Antibody against ISG15 (Center R87)

Product Images

Western blot analysis of ISG15 (arrow) using rabbit polyclonal ISG15 Antibody (Center R87) (Cat.#TA302035). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with ISG15 gene (Lane 2) (Origene Technologies).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This ISG15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-99 amino acids from the Central region of human ISG15.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17756 Da
• Gene Name: ISG15 ubiquitin-like modifier
• Database Link:
  NP_005092
  Entrez Gene 9636 Human
  UniProt ID P05161
• Background: ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega , but not IFN-gamma . ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.
• Synonyms: G1P2; hUCRP; IFI15; IMD38; IP17; UCRP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: RIG-I-like receptor signaling pathway