Bim (BCL2L11) (N-term) Rabbit Polyclonal Antibody

CAT#: SP2116P

Bim (BCL2L11) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of whole cell lysates from K562 (K) and A549 (A) cells probed with Rabbit anti BIM antibody Cat.-No SP2116P

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  Immunohistochemical staining of human skin with Rabbit anti BIM antibody Cat.-No SP2116P

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/1000. Detects a band of approximately 22kDa in K562 and A549 whole cell lysates.
  Immunohistochemistry on Paraffin Sections: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids 22 to 40 of Human Bim
• Specificity: This antibody is specific for Bcl-2 interacting mediator of cell death (Bim), also known as Bod.
• Formulation: State: Aff - Purified
  State: Liquid purified IgG fraction
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: BCL2 like 11
• Database Link:
  Entrez Gene 10018 Human
  UniProt ID O43521
• Background: Bim is a pro-apoptotic protein within the Bcl-2 family, which functions within the intrinsic apotosis pathway. Bim is known to interact with pro-survival proteins including Bcl-2, Bcl-XL and Bcl-w. Bim is expressed in a variety of cell types, where it is maintained in an inactive conformation by phosphorylation or through binding to the microtubule-associated dynein motor complex. Upon certain apoptotic stimuli, Bim is released from microtubules and mediates caspase-dependent apoptosis.
• Synonyms: Bcl2-L-11, BCL2L11, BIM, BimEL, BimL, BimS