CD71 (TFRC) Mouse Monoclonal Antibody [Clone ID: MEM-75]

CAT#: SM3104P

CD71 (TFRC) mouse monoclonal antibody, clone MEM-75, Purified

Product Images

Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Specifications

Product Data

• Clone Name: MEM-75
• Applications: FC, IF, IP
• Recommended Dilution: Flow cytometry: Recommended dilution: 0.5-4 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Pre-B cell line NALM-6
• Specificity: The antibody reacts with CD71 antigen (Transferrin Receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes.
  The antibody does not block binding of Transferrin to the Receptor.
• Formulation: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
  State: Purified
  State: Liquid purified Ig fraction (> 98% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Protein-A Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: transferrin receptor
• Database Link:
  Entrez Gene 7037 Human
  UniProt ID P02786
• Background: CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
• Synonyms: TfR1, p90, Transferrin receptor protein 1