SIT (SIT1) Mouse Monoclonal Antibody [Clone ID: SIT-01]

CAT#: SM3074P

SIT (SIT1) mouse monoclonal antibody, clone SIT-01, Purified

Product Images

Western blot of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained bymAbSIT-01 andgoat anti-mouse IgG (H+L)-HRPconjugate.

Specifications

Product Data

• Clone Name: SIT-01
• Applications: FC, IP, WB
• Recommended Dilution: Flow Cytometry: 1-5 μg/ml (intracellular staining).
  Immunoprecipitation.
  Western Blot: 1-2 μg/ml, reducing conditions. Western blot analysis demonstrated that SIT is expressed as an approximately 40 kDa protein that is reduced to approximately 20 kDa by endoglycosidase treatment.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bacterially produced recombinant intracellular fragment of human SIT
• Specificity: The antibody reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: signaling threshold regulating transmembrane adaptor 1
• Database Link:
  Entrez Gene 27240 Human
  UniProt ID Q9Y3P8
• Background: SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
• Synonyms: gp30/40; MGC125908; MGC125909; MGC125910; RP11-331F9.5; SIT

Reference Data

• Protein Families: Transmembrane