CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: MEM-28]

CAT#: SM3025P

CD45 (PTPRC) mouse monoclonal antibody, clone MEM-28, Purified

Product Images

Immunocytochemistry staining of Human peripheral blood mononuclear cell using anti-Human CD45 antibody (MEM-28, green). DNA visualized by DAPI (blue)

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  Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) purified, GAM-APC.

Specifications

Product Data

• Clone Name: MEM-28
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 1 µg/ml.
  Immunoprecipitation.
  Western Blot: 1 µg/ml.
  Positive Control: JURKAT Human leukemia T-cell lysate, Kg-1a Human leukemia cell lysate.
  Sample Preparation: buffer with laurylmaltoside, 2 x non-reducing SDS.
  Non-reducing conditions. SDS-PAGE (6% separating gel).
  Immunohistochemistry on Paraffin Sections: No pre-treatment of tissue sections is essential.
  Immunocytochemistry: 10 μg/ml (paraformaldehyde fixation can be used).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human thymocytes and T lymphocytes
• Specificity: The antibody clone MEM-28 reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
• Formulation: Phosphate Buffered Saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: protein tyrosine phosphatase, receptor type C
• Database Link:
  Entrez Gene 5788 Human
  UniProt ID P08575
• Background: CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200