CD11b (ITGAM) Mouse Monoclonal Antibody [Clone ID: MEM-174]

CAT#: SM3007P

CD11b (ITGAM) mouse monoclonal antibody, clone MEM-174, Purified

Product Images

Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC isolated from healthy donor. Lysate was subjected to affinity column chromatography using anti-human CD11b (MEM-174) immunosorbent. Eluted immunoprecipitate was resolved on 7.5% SDS-PAGE and stained with Coomasie Blue.

Specifications

Product Data

• Clone Name: MEM-174
• Applications: FC, IP
• Recommended Dilution: Flow Cytometry: 1 µg/ml.
  Positive Control: Peripheral Blood Lymphocytes.
  Immunoprecipitation
  Positive Control: Granulocytes, CD11b/CD18 transfectants.
  Application note: Immunoprecipitation with the antibody SM3007P was successfully performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human granulocytes
• Specificity: The antibody recognizes CD11b antigen (Mac-1), a 165 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
• Formulation: Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20 °C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha M
• Database Link:
  Entrez Gene 3684 Human
  UniProt ID P11215
• Background: CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1