Icam1 Mouse Monoclonal Antibody [Clone ID: 1A29]

CAT#: SM286F

Icam1 mouse monoclonal antibody, clone 1A29, FITC

Product Images

Staining of stimulated Rat spleen cells with FITC conjugated Mouse anti-Rat CD54 antibody

Specifications

Product Data

• Clone Name: 1A29
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat Ax cells (a rat HEV derived cell line).
• Specificity: This antibody recognizes the CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1).
  Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling.
  Functionally 1A29 inhibits homotypic aggregation of PHA blasts. We recommend SM286LE for this use.
• Formulation: PBS, pH 7.2, containing 1% BSA as stabiliser and 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified IgG fraction
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: intercellular adhesion molecule 1
• Database Link:
  Entrez Gene 25464 Rat
  UniProt ID Q00238
• Background: CD54 cell surface antigen, also known as the intercellular adhesion molecule-1 (ICAM-1), is a 90 kD adhesion molecule belonging to the immunoglobulin superfamily. This is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system.
• Synonyms: ICAM-1