Cd4 (Domain 1) Mouse Monoclonal Antibody [Clone ID: W3/25]
Other products for "Cd4"
Specifications
Product Data | |
Clone Name | W3/25 |
Applications | FC, FN, IHC |
Recommended Dilution | Flow Cytometry: Use 10 µl of 1/100-1/200 diluted antibody to label 106 cells in 100 µl. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: PLP fixation is recommended for optimal results, See Whiteland et al. for details. |
Reactivities | Rat |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Rat Thymocyte Membrane Glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS-1 mouse myeloma cell line. |
Specificity | This antibody recognises the CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde). |
Formulation | PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | Cd4 molecule |
Database Link | |
Background | CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase. |
Synonyms | T-cell surface antigen T4/Leu-3 |
Reference Data |
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