CD62P (SELP) Mouse Monoclonal Antibody [Clone ID: Psel.KO.2.7]

CAT#: SM2261P

CD62P (SELP) mouse monoclonal antibody, clone Psel.KO.2.7, Purified

Product Images

Staining of thrombin activated human platelets with antibody.

Specifications

Product Data

• Clone Name: Psel.KO.2.7
• Applications: FC, IHC, IP
• Recommended Dilution: Immunoprecipitation.
  Flow Cytometry: Use 10 µl of 1/25-1/50 diluted antibody to label 10⁶ cells in 100 µl.
  Immunohistology on Frozen Sections: 1/200 (See Reference 1).
• Reactivities: Bovine, Equine, Feline, Goat, Human, Mouse, Rat, Sheep
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: P-selectin transfected 300.19 cells.
• Specificity: This antibody recognises the CD62P cell surface antigen.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: selectin P
• Database Link:
  Entrez Gene 6403 Human
  UniProt ID P16109
• Background: CD62P is a 140 kD glycoprotein expressed by activated platelets and endothelial cells. It plays an important role in adhesive processes between leucocytes and endothelial cells.
  CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.
• Synonyms: SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3