HLA Class II DR Rat Monoclonal Antibody [Clone ID: YE2/36-HLK]
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Specifications
Product Data | |
Clone Name | YE2/36-HLK |
Applications | FC, IHC |
Recommended Dilution | Flow Cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on frozen and paraffin sections: This clone has been described reacting with paraffin embedded material following PLP (periodate-lysineparaformaldehyde) fixation. |
Reactivities | Human, Mouse |
Host | Rat |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | EHR-B Ramos cells. Spleen cells from immunised AO rats were fused with cells of the rat Y3 Ag1.2.3 myeloma cell line. |
Specificity | This antibody recognises a monomorphic determinant of HLA-DR antigen (MHC class II). Clone YE2/36 has also been reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB. |
Formulation | State: Purified State: Liquid purified IgG containing 0.09% Sodium Azide |
Concentration | lot specific |
Purification | Affinity chromatography on Protein G |
Conjugation | Unconjugated |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Background | HLA DR is expressed by B lymphocytes, activated T cells and by monocytes. |
Synonyms | HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR |
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