CD59 Rat Monoclonal Antibody [Clone ID: YTH53.1]

CAT#: SM1768R

CD59 rat monoclonal antibody, clone YTH53.1, PE

Specifications

Product Data

• Clone Name: YTH53.1
• Applications: FC
• Recommended Dilution: Flow cytometry: We do not recommend using this antibody in lysed whole blood techniques due to inhibition of staining caused by CD59 expression on erythrocytes.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human Peripheral Blood T cells. Spleen cells from immunised DA rat fused with the Y3/Ag1.2.3 meloma cell line.
• Specificity: Clustered at the fourth leucocyte typing workshop to designation CD59. YTH53.1 reacts with CD59 transfectants. It enhances antibody-mediated lysis by homologous complement and reacts with GPI-linked antigen of 18-20kD size which is broadly expressed on human leucocytes and erythrocytes. Putative ligand for CD2.
• Formulation: PBS, pH7.2 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: PE
  State: Lyophilized purified IgG
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1 ml distilled water
• Purification: Ion exchange chromatography
• Conjugation: PE
• Storage: Prior to and following reconstitution store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD59 molecule
• Database Link:
  Entrez Gene 966 Human
  UniProt ID P13987
• Background: CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. It is a potent inhibitor of the complement membrane attack complex action. It acts by binding to the C8 and/or C9 complements of the membrane attack complex, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is also involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
• Synonyms: MAC-inhibitory protein, Protectin, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MSK21, MACIF, MAC-IP, MIRL, HRF20, HRF-20