CD49b (ITGA2) Mouse Monoclonal Antibody [Clone ID: AK7]

CAT#: SM1125P

CD49b (ITGA2) mouse monoclonal antibody, clone AK7, Purified

Product Images

Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD49b.

Specifications

Product Data

• Clone Name: AK7
• Applications: FC
• Recommended Dilution: Flow cytometry: 1/50 - 1/100; use 10 µl of the suggested working dilution to label 10e6 cells in 100µl.
  Immunohistochemistry on frozen sections: 1/500 - 1/1000. Recommended positive control tissue: Tonsil.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises the integrin alpha2 subunit, which is a 160kD glycoprotein that non-covalently associates with the 130kD integrin beta1 subunit to form the VLA-2 complex. CD49b is expressed by platelets, long term cultivated T cells, endothelial cells, monocytes and most adherent cell lines. Cross reacts with Cynomolgus monkey (3), Baboon and Rhesus monkey.
• Formulation: PBS containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha 2
• Database Link:
  Entrez Gene 3673 Human
  UniProt ID P17301
• Background: CD49b is a 160kD glycoprotein that non-covalently associates with the 130kD integrin beta 1 subunit to form the VLA-2 complex. CD49b is expressed by platelets, long term cultivated T cells, approximately 50% of monocytes and most adherent cell lines. MCA743 inhibits cell attachment to collagen.
• Synonyms: Integrin alpha-2, ITGA-2, VLA-2 alpha, VLA2, GPIa, Collagen Receptor