CD33 Mouse Monoclonal Antibody [Clone ID: WM53]

CAT#: SM1103R

CD33 mouse monoclonal antibody, clone WM53, PE

Product Images

Staining of human peripheral blood monocytes with Mouse Anti Human CD33 antibody -RPE

Specifications

Product Data

• Clone Name: WM53
• Applications: FC
• Recommended Dilution: Flow Ccytometry: Neat-1/5; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human AML cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
• Specificity: This antibody recognises the CD33 cell surface glycoprotein.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with distilled water.
• Purification: Affinity chromatography on Protein G.
• Conjugation: PE
• Storage: Prior to and following reconstitution store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD33 molecule
• Database Link:
  Entrez Gene 945 Human
  UniProt ID P20138
• Background: CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
  Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67kDa M.W. surface antigen.
  CD33 is a 67kD molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.
• Synonyms: Siglec-3, gp67